{"id":257,"date":"2025-05-04T05:50:18","date_gmt":"2025-05-04T05:50:18","guid":{"rendered":"https:\/\/thuoc115.com\/suckhoe\/?p=257"},"modified":"2025-05-04T05:56:18","modified_gmt":"2025-05-04T05:56:18","slug":"chiet-xuat-tanshinones-tu-dan-sam-bang-phuong-phap-chiet-xuat-diem-may-cpe","status":"publish","type":"post","link":"https:\/\/thuoc115.com\/suckhoe\/chiet-xuat-tanshinones-tu-dan-sam-bang-phuong-phap-chiet-xuat-diem-may-cpe\/","title":{"rendered":"Chi\u1ebft Xu\u1ea5t Tanshinones t\u1eeb \u0110an s\u00e2m b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t \u0111i\u1ec3m m\u00e2y (CPE)"},"content":{"rendered":"\n

\u0110\u1ec3 d\u1ecbch, hi\u1ec7u ch\u1ec9nh v\u00e0 l\u1eadp ra danh m\u1ee5c c\u00e1c b\u00e0i vi\u1ebft nghi\u00ean c\u1ee9u n\u00e0y r\u1ea5t t\u1ed1n th\u1eddi gian v\u00e0 t\u1ed1n k\u00e9m chi ph\u00ed. R\u1ea5t vui \u0111\u01b0\u1ee3c anh em \u0111\u1ecdc v\u00e0 xem x\u00e9t, nh\u01b0ng c\u00f3 th\u1ec3 vui l\u00f2ng c\u00e1c b\u00e1c s\u0129, d\u01b0\u1ee3c s\u0129, l\u01b0\u01a1ng y v\u00e0 th\u1ea7y thu\u1ed1c y h\u1ecdc c\u1ed5 truy\u1ec1n \u1ee7ng h\u1ed9 mua c\u00e1c s\u1ea3n ph\u1ea9m do ch\u00fang t\u00f4i nghi\u00ean c\u1ee9u, chi\u1ebft xu\u1ea5t \u0111\u1ec3 \u1ee7ng h\u1ed9 kinh ph\u00ed nh\u1eb1m c\u00f3 \u0111i\u1ec1u ki\u1ec7n ph\u1ee5c v\u1ee5 t\u1ed1t h\u01a1n sau n\u00e0y<\/p>\n\n\n\n

Ch\u00fang t\u00f4i nh\u1eadn chi\u1ebft xu\u1ea5t \u0110an s\u00e2m, \u0111an s\u00e2m nano v\u1edbi quy tr\u00ecnh chu\u1ea9n v\u1edbi hi\u1ec7u su\u1ea5t cao nh\u1ea5t, t\u1ea1o ra dung d\u1ecbch nano \u0110an s\u00e2m ho\u1eb7c cao \u0111\u1eb7c, cao l\u1ecfng v\u1edbi ch\u1ea5t l\u01b0\u1ee3ng t\u1ed1i \u01b0u ch\u1ec9 t\u1eeb 1kg (t\u1ef7 l\u1ec7 1:10)<\/p>\n\n\n\n

Gi\u1ea3i th\u00edch c\u00e1c th\u00e0nh ph\u1ea7n trong \u0110an s\u00e2m<\/a><\/p>\n\n\n\n

Dihydrotanshinone I: Ph\u01b0\u01a1ng ph\u00e1p CPE gi\u00fap t\u0103ng hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t l\u00ean 4,55% so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t truy\u1ec1n th\u1ed1ng.<\/p>\n\n\n\n

Cryptotanshinone: T\u1ef7 l\u1ec7 chi\u1ebft xu\u1ea5t t\u0103ng 8,32% khi s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p CPE.<\/p>\n\n\n\n

Tanshinone I: Ph\u01b0\u01a1ng ph\u00e1p CPE \u0111\u1ea1t hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t cao h\u01a1n 15,77% so v\u1edbi chi\u1ebft xu\u1ea5t truy\u1ec1n th\u1ed1ng.<\/p>\n\n\n\n

Tanshinone IIA: T\u1ef7 l\u1ec7 chi\u1ebft xu\u1ea5t t\u0103ng 6,81% khi s\u1eed d\u1ee5ng ph\u01b0\u01a1ng ph\u00e1p CPE.<\/p>\n\n\n\n

T\u00f3m l\u1ea1i, ph\u01b0\u01a1ng ph\u00e1p CPE kh\u00f4ng ch\u1ec9 t\u0103ng hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t c\u1ee7a c\u00e1c h\u1ee3p ch\u1ea5t quan tr\u1ecdng t\u1eeb Salvia miltiorrhiza m\u00e0 c\u00f2n gi\u1ea3m b\u1edbt s\u1ef1 ph\u1ee5 thu\u1ed9c v\u00e0o c\u00e1c dung m\u00f4i h\u1eefu c\u01a1, l\u00e0m cho ph\u01b0\u01a1ng ph\u00e1p n\u00e0y th\u00e2n thi\u1ec7n v\u1edbi m\u00f4i tr\u01b0\u1eddng h\u01a1n.<\/p>\n\n\n\n

Gi\u1edbi thi\u1ec7u v\u1ec1 \u0110an s\u00e2m l\u00e0 g\u00ec<\/p>\n\n\n\n

Salvia miltiorrhiza (\u0110an s\u00e2m) l\u00e0 m\u1ed9t chi thu\u1ed9c h\u1ecd Lamiaceae – H\u1ecd hoa m\u00f4i, \u0111\u00e3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng truy\u1ec1n th\u1ed1ng \u1edf c\u00e1c qu\u1ed1c gia ch\u00e2u \u00c1 nh\u01b0 Trung Qu\u1ed1c, H\u00e0n Qu\u1ed1c v\u00e0 Nh\u1eadt B\u1ea3n, Vi\u1ec7t Nam [1]. SM ch\u1ee9a h\u01a1n 100 h\u1ee3p ch\u1ea5t ho\u1ea1t t\u00ednh, \u0111\u01b0\u1ee3c ph\u00e2n lo\u1ea1i th\u00e0nh hai nh\u00f3m ch\u00ednh: c\u00e1c th\u00e0nh ph\u1ea7n axit phenolic tan trong n\u01b0\u1edbc v\u00e0 c\u00e1c tanshinone kh\u00f4ng tan trong n\u01b0\u1edb<\/strong>c [2]. Nh\u00f3m tanshinone ch\u1ee7 y\u1ebfu bao g\u1ed3m c\u00e1c h\u1ee3p ch\u1ea5t lipophilic phenanthrene\u2013quinone v\u00e0 c\u00e1c d\u1eabn xu\u1ea5t c\u1ee7a n\u00f3 [3], bao g\u1ed3m dihydrotanshinone I (DHTS), cryptotanshinone (CT), tanshinone I (Tan I) v\u00e0 tanshinone IIA (Tan IIA). <\/p>\n\n\n\n

Ch\u00fa th\u00edch : Tanshione IIA l\u00e0 ch\u1ea5t quan tr\u1ecdng nh\u1ea5t, \u0111\u00e3 \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t tinh khi\u1ebft \u0111\u1ec3 ti\u00eam cho b\u1ec7nh nh\u00e2n \u1edf m\u1ed9t s\u1ed1 qu\u1ed1c gia \u0111\u00e3 ph\u00ea duy\u1ec7t ch\u1ea5t n\u00e0y trong \u0111i\u1ec1u tr\u1ecb<\/p>\n\n\n\n

Nh\u1eefng ch\u1ea5t chuy\u1ec3n h\u00f3a th\u1ee9 c\u1ea5p n\u00e0y ch\u1ee7 y\u1ebfu t\u00edch t\u1ee5 trong r\u1ec5 v\u00e0 th\u1ec3 hi\u1ec7n nhi\u1ec1u t\u00e1c d\u1ee5ng d\u01b0\u1ee3c l\u00fd kh\u00e1c nhau, nh\u01b0 t\u00ednh kh\u00e1ng khu\u1ea9n, ch\u1ed1ng oxy h\u00f3a v\u00e0 ch\u1ed1ng ung th\u01b0, khi\u1ebfn ch\u00fang tr\u1edf th\u00e0nh nh\u1eefng \u1ee9ng vi\u00ean h\u1ee9a h\u1eb9n trong \u0111i\u1ec1u tr\u1ecb c\u00e1c b\u1ec7nh nh\u01b0 b\u1ec7nh tim m\u1ea1ch v\u00e0 b\u1ec7nh l\u00fd m\u1ea1ch m\u00e1u n\u00e3o [4,5]. \u0110\u1eb7c bi\u1ec7t, do c\u00f3 ngu\u1ed3n g\u1ed1c t\u1ef1 nhi\u00ean, c\u00e1c tanshinone \u0111\u00e3 \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o nhi\u1ec1u c\u00f4ng th\u1ee9c ch\u1ebf ph\u1ea9m nh\u01b0 vi\u00ean n\u00e9n, ti\u00eam v\u00e0 thu\u1ed1c m\u1ee1 k\u1ebft h\u1ee3p v\u1edbi y h\u1ecdc hi\u1ec7n \u0111\u1ea1i, mang l\u1ea1i c\u00e1c l\u1ef1a ch\u1ecdn \u0111i\u1ec1u tr\u1ecb m\u1edbi v\u00e0 gi\u00e1 tr\u1ecb c\u00f4ng nghi\u1ec7p \u0111\u00e1ng k\u1ec3 nh\u01b0 c\u00e1c v\u1eadt li\u1ec7u s\u1ea3n ph\u1ea9m t\u1ef1 nhi\u00ean c\u00f3 gi\u00e1 tr\u1ecb cao [6]. Tuy nhi\u00ean, m\u1eb7c d\u00f9 c\u00f3 gi\u00e1 tr\u1ecb cao, n\u0103ng su\u1ea5t chi\u1ebft xu\u1ea5t tanshinone v\u1eabn c\u00f2n th\u1ea5p v\u00e0 hi\u1ec7n nay \u0111ang c\u00f3 nghi\u00ean c\u1ee9u \u0111\u1ec3 c\u1ea3i thi\u1ec7n vi\u1ec7c s\u1eed d\u1ee5ng hi\u1ec7u qu\u1ea3 ch\u00fang [7,8].<\/p>\n\n\n\n

Ph\u01b0\u01a1ng ph\u00e1p truy\u1ec1n th\u1ed1ng \u0111\u1ec3 chi\u1ebft xu\u1ea5t tanshinone s\u1eed d\u1ee5ng dung m\u00f4i h\u1eefu c\u01a1 nh\u01b0 ethanol v\u00e0 methanol. Tuy nhi\u00ean, vi\u1ec7c \u00e1p d\u1ee5ng nh\u1eefng dung m\u00f4i n\u00e0y trong c\u00e1c ng\u00e0nh c\u00f4ng nghi\u1ec7p nh\u01b0 th\u1ef1c ph\u1ea9m v\u00e0 d\u01b0\u1ee3c ph\u1ea9m b\u1ecb h\u1ea1n ch\u1ebf do \u0111\u1ed9c t\u00ednh v\u00e0 kh\u1ea3 n\u0103ng ch\u00e1y cao c\u1ee7a ch\u00fang. Th\u00eam v\u00e0o \u0111\u00f3, ngay c\u1ea3 khi s\u1eed d\u1ee5ng dung m\u00f4i, c\u00e1c b\u01b0\u1edbc chi\u1ebft xu\u1ea5t v\u1eabn c\u1ea7n thi\u1ebft \u0111\u1ec3 lo\u1ea1i b\u1ecf ch\u00fang sau khi chi\u1ebft, v\u00e0 cu\u1ed1i c\u00f9ng, \u0111i\u1ec1u n\u00e0y l\u00e0m t\u0103ng chi ph\u00ed s\u1ea3n xu\u1ea5t. H\u01a1n n\u1eefa, dung m\u00f4i d\u01b0 c\u00f3 th\u1ec3 v\u00f4 t\u00ecnh c\u00f2n l\u1ea1i, g\u00e2y lo ng\u1ea1i v\u1ec1 ch\u1ea5t l\u01b0\u1ee3ng v\u00e0 \u0111\u1ed9 an to\u00e0n c\u1ee7a s\u1ea3n ph\u1ea9m [9]. H\u01b0\u1edbng d\u1eabn c\u1ee7a H\u1ed9i \u0111\u1ed3ng Qu\u1ed1c t\u1ebf v\u1ec1 H\u00e0i h\u00f2a (ICH) \u0111\u1eb7t gi\u1edbi h\u1ea1n \u0111\u1ed1i v\u1edbi dung m\u00f4i d\u01b0 trong dung m\u00f4i h\u1eefu c\u01a1, quy \u0111\u1ecbnh t\u1ed1i \u0111a 30 mg\/L \u0111\u1ed1i v\u1edbi methanol v\u00e0 50 mg\/ng\u00e0y \u0111\u1ed1i v\u1edbi ethanol [10]. Trong khi T\u1ed5 ch\u1ee9c N\u00f4ng l\u01b0\u01a1ng Li\u00ean H\u1ee3p Qu\u1ed1c (FAO) kh\u00f4ng quy \u0111\u1ecbnh v\u1ec1 ethanol, h\u1ecd khuy\u1ebfn ngh\u1ecb gi\u1edbi h\u1ea1n 10 mg\/kg \u0111\u1ed1i v\u1edbi methanol trong h\u1ea7u h\u1ebft c\u00e1c lo\u1ea1i th\u1ef1c ph\u1ea9m [11].<\/p>\n\n\n\n

C\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t thay th\u1ebf bao g\u1ed3m ng\u00e2m, th\u1ea9m th\u1ea5u v\u00e0 chi\u1ebft xu\u1ea5t si\u00eau \u00e2m [12]; chi\u1ebft xu\u1ea5t si\u00eau \u00e2m li\u00ean t\u1ee5c v\u1edbi \u0111\u1ea7u d\u00f2 si\u00eau \u00e2m c\u01b0\u1eddng \u0111\u1ed9 cao (CUAE-HIUP) [13]; chi\u1ebft xu\u1ea5t b\u1eb1ng ch\u1ea5t l\u1ecfng si\u00eau t\u1edbi h\u1ea1n (SFE) [14]; chi\u1ebft xu\u1ea5t b\u1eb1ng ch\u1ea5t l\u1ecfng \u00e1p su\u1ea5t [15]; chi\u1ebft xu\u1ea5t h\u1ed7 tr\u1ee3 h\u1ed3ng ngo\u1ea1i [16]; v\u00e0 chi\u1ebft xu\u1ea5t b\u1eb1ng ch\u1ea5t l\u1ecfng ion h\u00f3a v\u1edbi \u00e1p su\u1ea5t c\u1ef1c cao [17]. Tuy nhi\u00ean, v\u1eabn c\u00f2n c\u1ea7n thi\u1ebft c\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t \u0111\u01a1n gi\u1ea3n v\u00e0 an to\u00e0n h\u01a1n, v\u00ec nhi\u1ec1u k\u1ef9 thu\u1eadt hi\u1ec7n t\u1ea1i y\u00eau c\u1ea7u thi\u1ebft b\u1ecb \u0111\u1eaft ti\u1ec1n ho\u1eb7c li\u00ean quan \u0111\u1ebfn c\u00e1c quy tr\u00ecnh ph\u1ee9c t\u1ea1p c\u00f3 th\u1ec3 l\u00e0m t\u0103ng chi ph\u00ed s\u1ea3n xu\u1ea5t.<\/p>\n\n\n\n

Chi\u1ebft xu\u1ea5t \u0111i\u1ec3m m\u00e2y (CPE) l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt chi\u1ebft xu\u1ea5t l\u1ecfng\u2013l\u1ecfng s\u1eed d\u1ee5ng nguy\u00ean l\u00fd r\u1eb1ng dung d\u1ecbch ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, khi \u0111\u01b0\u1ee3c \u0111un n\u00f3ng tr\u00ean \u0111i\u1ec3m m\u00e2y c\u1ee7a n\u00f3, s\u1ebd khi\u1ebfn c\u00e1c micelle c\u1ee7a ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t k\u1ebft t\u1ee5 l\u1ea1i. \u0110i\u1ec1u n\u00e0y l\u00e0m dung d\u1ecbch tr\u1edf n\u00ean \u0111\u1ee5c v\u00e0 t\u00e1ch th\u00e0nh m\u1ed9t l\u1edbp gi\u00e0u ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t v\u00e0 m\u1ed9t l\u1edbp n\u01b0\u1edbc. C\u00e1c micelle \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh khi c\u00e1c ph\u00e2n t\u1eed ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ef1 \u0111\u1ed9ng k\u1ebft t\u1ee5 trong dung d\u1ecbch d\u01b0\u1edbi c\u00e1c \u0111i\u1ec1u ki\u1ec7n nh\u1ea5t \u0111\u1ecbnh, nh\u01b0 nhi\u1ec7t \u0111\u1ed9 v\u00e0 n\u1ed3ng \u0111\u1ed9 c\u1ee5 th\u1ec3 [18]. \u0110u\u00f4i k\u1ef5 n\u01b0\u1edbc c\u1ee7a ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ea1o th\u00e0nh ph\u1ea7n trong c\u1ee7a micelle, c\u00f3 kh\u1ea3 n\u0103ng gi\u1eef c\u00e1c ch\u1ea5t k\u1ef5 n\u01b0\u1edbc, trong khi \u0111\u1ea7u \u01b0a n\u01b0\u1edbc gi\u1eef v\u1eefng micelle trong l\u1edbp n\u01b0\u1edbc [19]. Trong qu\u00e1 tr\u00ecnh CPE, c\u00e1c ch\u1ea5t ph\u00e2n t\u00edch b\u1ecb gi\u1eef trong c\u00e1c micelle v\u00e0 \u0111\u01b0\u1ee3c t\u1eadp trung v\u00e0o l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t [20]. L\u1edbp n\u01b0\u1edbc sau \u0111\u00f3 \u0111\u01b0\u1ee3c lo\u1ea1i b\u1ecf, cho ph\u00e9p t\u00e1ch v\u00e0 c\u00f4 \u0111\u1eb7c c\u00e1c h\u1ee3p ch\u1ea5t m\u1ee5c ti\u00eau. CPE, c\u00f2n \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 chi\u1ebft xu\u1ea5t d\u1ef1a tr\u00ean ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, k\u1ef9 thu\u1eadt c\u00f4 \u0111\u1eb7c l\u1ecfng ho\u1eb7c chi\u1ebft xu\u1ea5t qua trung gian micelle [21], \u0111\u00e3 \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng \u0111\u1ec3 chi\u1ebft xu\u1ea5t c\u00e1c h\u1ee3p ch\u1ea5t h\u1eefu c\u01a1 t\u1eeb th\u1ef1c ph\u1ea9m, kim lo\u1ea1i n\u1eb7ng t\u1eeb n\u01b0\u1edbc, v\u00e0 c\u00e1c ch\u1ea5t sinh h\u1ecdc t\u1eeb th\u1ef1c v\u1eadt [22\u201324]. Ph\u01b0\u01a1ng ph\u00e1p n\u00e0y \u0111\u01a1n gi\u1ea3n, hi\u1ec7u qu\u1ea3 v\u1ec1 chi ph\u00ed v\u00e0 th\u00e2n thi\u1ec7n v\u1edbi m\u00f4i tr\u01b0\u1eddng, v\u00ec n\u00f3 gi\u1ea3m thi\u1ec3u ho\u1eb7c lo\u1ea1i b\u1ecf vi\u1ec7c s\u1eed d\u1ee5ng dung m\u00f4i h\u1eefu c\u01a1 [20,25]. C\u00e1c ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t kh\u00f4ng ion nh\u01b0 Triton X-100, Triton X-114 v\u00e0 Tween 80 th\u01b0\u1eddng \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong CPE. C\u00e1c nghi\u00ean c\u1ee9u tr\u01b0\u1edbc \u0111\u00e2y \u0111\u00e3 s\u1eed d\u1ee5ng c\u00e1c ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ed5ng h\u1ee3p nh\u01b0 Genapol X-080 ho\u1eb7c Triton X-100 \u0111\u1ec3 chi\u1ebft xu\u1ea5t tanshinone t\u1eeb SM [26,27]. M\u1eb7c d\u00f9 c\u00e1c ch\u1ea5t n\u00e0y \u0111\u01b0\u1ee3c FDA (C\u1ee5c Qu\u1ea3n l\u00fd Th\u1ef1c ph\u1ea9m v\u00e0 D\u01b0\u1ee3c ph\u1ea9m Hoa K\u1ef3) ph\u00ea duy\u1ec7t l\u00e0 c\u00f3 th\u1ec3 \u0103n \u0111\u01b0\u1ee3c, ch\u00fang kh\u00f4ng ph\u1ea3i l\u00e0 ngu\u1ed3n g\u1ed1c t\u1ef1 nhi\u00ean. Ng\u01b0\u1ee3c l\u1ea1i, lecithin, m\u1ed9t ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ef1 nhi\u00ean c\u00f3 ngu\u1ed3n g\u1ed1c t\u1eeb c\u00e1c ngu\u1ed3n nh\u01b0 hoa h\u01b0\u1edbng d\u01b0\u01a1ng, \u0111\u1eadu n\u00e0nh v\u00e0 tr\u1ee9ng, \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng r\u1ed9ng r\u00e3i trong c\u00e1c ng\u00e0nh th\u1ef1c ph\u1ea9m, d\u01b0\u1ee3c ph\u1ea9m v\u00e0 m\u1ef9 ph\u1ea9m nh\u1edd t\u00ednh kh\u00f4ng \u0111\u1ed9c v\u00e0 kh\u1ea3 n\u0103ng t\u01b0\u01a1ng th\u00edch sinh h\u1ecdc. Lecithin c\u0169ng \u0111\u01b0\u1ee3c FDA ph\u00e2n lo\u1ea1i l\u00e0 GRAS (\u0110\u01b0\u1ee3c c\u00f4ng nh\u1eadn r\u1ed9ng r\u00e3i l\u00e0 an to\u00e0n) [28]. Th\u00eam v\u00e0o \u0111\u00f3, lecithin \u0111\u01b0\u1ee3c thi\u1ebft k\u1ebf nh\u01b0 m\u1ed9t ph\u1ee5 gia th\u1ef1c ph\u1ea9m trong Li\u00ean minh Ch\u00e2u \u00c2u (EU) v\u1edbi m\u00e3 E322 v\u00e0 c\u00f3 th\u1ec3 \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng m\u00e0 kh\u00f4ng c\u00f3 gi\u1edbi h\u1ea1n v\u1ec1 s\u1ed1 l\u01b0\u1ee3ng [28,29]. C\u00e1c nghi\u00ean c\u1ee9u v\u1ec1 vi\u1ec7c s\u1eed d\u1ee5ng lecithin trong chi\u1ebft xu\u1ea5t h\u1ee3p ch\u1ea5t t\u1eeb th\u1ef1c v\u1eadt ch\u1ee7 y\u1ebfu t\u1eadp trung v\u00e0o c\u00e1c s\u1ea3n ph\u1ea9m ph\u1ee5 t\u1eeb th\u1ef1c ph\u1ea9m, nh\u01b0 phenolic t\u1eeb ch\u1ea5t th\u1ea3i \u00f4 liu, h\u1ee3p ch\u1ea5t phenolic t\u1eeb ch\u1ea5t th\u1ea3i r\u01b0\u1ee3u vang v\u00e0 carotenoid t\u1eeb n\u01b0\u1edbc th\u1ea3i c\u00e0 chua [28,30,31]. Tuy nhi\u00ean, c\u00f3 \u00edt nghi\u00ean c\u1ee9u v\u1ec1 vi\u1ec7c \u1ee9ng d\u1ee5ng lecithin tr\u1ef1c ti\u1ebfp \u0111\u1ec3 chi\u1ebft xu\u1ea5t c\u00e1c h\u1ee3p ch\u1ea5t c\u1ee5 th\u1ec3 t\u1eeb th\u1ef1c v\u1eadt.<\/p>\n\n\n\n

V\u1edbi nhu c\u1ea7u ng\u00e0y c\u00e0ng t\u0103ng v\u1ec1 tanshinone \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t t\u1eeb SM, c\u00f3 m\u1ed9t nhu c\u1ea7u c\u1ea5p thi\u1ebft v\u1ec1 c\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t hi\u1ec7u qu\u1ea3, t\u1ed1i \u0111a h\u00f3a n\u0103ng su\u1ea5t v\u00e0 b\u1ea3o t\u1ed3n ho\u1ea1t t\u00ednh sinh h\u1ecdc. Nghi\u00ean c\u1ee9u n\u00e0y nh\u1eb1m t\u1ed1i \u01b0u h\u00f3a ph\u01b0\u01a1ng ph\u00e1p CPE s\u1eed d\u1ee5ng lecithin, m\u1ed9t ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ef1 nhi\u00ean, nh\u01b0 m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p thay th\u1ebf dung m\u00f4i h\u1eefu c\u01a1 \u0111\u1ec3 chi\u1ebft xu\u1ea5t tanshinone, c\u00e1c h\u1ee3p ch\u1ea5t k\u1ef5 n\u01b0\u1edbc c\u00f3 trong SM. Chi\u1ebft xu\u1ea5t CPE t\u1ed1i \u01b0u theo t\u1eebng b\u01b0\u1edbc \u0111\u01b0\u1ee3c so s\u00e1nh v\u1edbi m\u1eabu chi\u1ebft xu\u1ea5t n\u01b0\u1edbc kh\u00f4ng c\u00f3 CPE \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t qua ph\u00e2n t\u00edch th\u00e0nh ph\u1ea7n.<\/p>\n\n\n\n

    \n
  1. V\u1eadt li\u1ec7u v\u00e0 ph\u01b0\u01a1ng ph\u00e1p<\/strong><\/li>\n<\/ol>\n\n\n\n

    2.1. H\u00f3a ch\u1ea5t<\/strong><\/p>\n\n\n\n

    Lecithin r\u1eafn chi\u1ebft xu\u1ea5t t\u1eeb \u0111\u1eadu n\u00e0nh, c\u00f9ng v\u1edbi c\u00e1c chu\u1ea9n \u0111\u1ec3 ph\u00e2n t\u00edch HPLC, bao g\u1ed3m dihydrotanshinone I (DHTS), cryptotanshinone (CT), tanshinone I (Tan I) v\u00e0 tanshinone IIA (Tan IIA) \u0111\u01b0\u1ee3c mua t\u1eeb Sigma-Aldrich (St. Louis, MO, USA). NaCl, axit citric anhydrous v\u00e0 methanol \u0111\u01b0\u1ee3c cung c\u1ea5p t\u1eeb DAEJUNG (Siheung, C\u1ed9ng h\u00f2a H\u00e0n Qu\u1ed1c). Gi\u1ea5y l\u1ecdc (No. 6) \u0111\u01b0\u1ee3c mua t\u1eeb Whatman International Ltd. (Maidstone, UK), v\u00e0 c\u00e1c b\u1ed9 l\u1ecdc \u1ed1ng ti\u00eam (13JP020AN, 13HP020AN, 0.2\u00b5m) \u0111\u01b0\u1ee3c mua t\u1eeb Advantec Mfs. Inc. (Dublin, CA, USA). Acetonitrile v\u00e0 n\u01b0\u1edbc c\u1ea5p HPLC \u0111\u01b0\u1ee3c cung c\u1ea5p b\u1edfi DUKSAN (Ansan, C\u1ed9ng h\u00f2a H\u00e0n Qu\u1ed1c), v\u00e0 axit acetic glacier \u0111\u01b0\u1ee3c mua t\u1eeb J.T. Baker (Phillipsburg, NJ, USA).<\/p>\n\n\n\n

    2.2. Ti\u1ec1n X\u1eed L\u00fd M\u1eabu<\/strong><\/p>\n\n\n\n

    C\u00e1c m\u1eabu \u0110an s\u00e2m t\u1eeb gi\u1ed1ng H\u00e0n Qu\u1ed1c \u2018Dasan\u2019 \u0111\u01b0\u1ee3c tr\u1ed3ng v\u00e0 thu ho\u1ea1ch v\u00e0o th\u00e1ng 4 n\u0103m 2021 b\u1edfi Ph\u00f2ng Chuy\u00ean M\u00f4n Nh\u00e2n S\u00e2m c\u1ee7a C\u01a1 Quan Ph\u00e1t Tri\u1ec3n N\u00f4ng Th\u00f4n \u1edf Eumsung, H\u00e0n Qu\u1ed1c. C\u00e1c m\u1eabu \u0111\u01b0\u1ee3c s\u1ea5y \u1edf nhi\u1ec7t \u0111\u1ed9 55\u00b0C v\u00e0 xay th\u00e0nh b\u1ed9t m\u1ecbn, sau \u0111\u00f3 \u0111\u01b0\u1ee3c b\u1ea3o qu\u1ea3n \u1edf nhi\u1ec7t \u0111\u1ed9 4\u00b0C cho \u0111\u1ebfn khi c\u1ea7n s\u1eed d\u1ee5ng cho c\u00e1c th\u00ed nghi\u1ec7m. \u0110\u1ec3 ti\u1ebfn h\u00e0nh qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t, 1 g m\u1eabu \u0111\u01b0\u1ee3c c\u00e2n v\u00e0 th\u00eam v\u00e0o dung d\u1ecbch n\u01b0\u1edbc c\u00f3 ch\u1ee9a 5% lecithin (w\/v). T\u1ef7 l\u1ec7 dung m\u00f4i \u0111\u01b0\u1ee3c \u0111\u00e1nh gi\u00e1 trong kho\u1ea3ng t\u1eeb 20 mL \u0111\u1ebfn 80 mL \u0111\u1ec3 x\u00e1c \u0111\u1ecbnh t\u1ef7 l\u1ec7 r\u1eafn\/l\u1ecfng t\u1ed1i \u01b0u. Sau khi khu\u1ea5y \u0111\u1ec1u, qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng trong 40 ph\u00fat b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t h\u1ed7 tr\u1ee3 si\u00eau \u00e2m (UAE, 120 V, 60 Hz, UCP-20, JeioTech, Daejeon, H\u00e0n Qu\u1ed1c). Dung d\u1ecbch l\u1ecfng trong su\u1ed1t \u0111\u01b0\u1ee3c t\u00e1ch b\u1eb1ng ly t\u00e2m (Vision, Daejeon, H\u00e0n Qu\u1ed1c) \u1edf t\u1ed1c \u0111\u1ed9 3500 rpm trong 15 ph\u00fat \u0111\u1ec3 thu \u0111\u01b0\u1ee3c d\u1ecbch l\u1ecfng trong su\u1ed1t.<\/p>\n\n\n\n

    \u0110\u1ec3 so s\u00e1nh v\u1edbi c\u00e1c ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t \u0110an s\u00e2m kh\u00e1c sau khi t\u1ed1i \u01b0u h\u00f3a \u0111i\u1ec1u ki\u1ec7n CPE, c\u00e1c th\u1ec3 t\u00edch dung m\u00f4i gi\u1ed1ng nhau (g m\u1eabu\/mL dung m\u00f4i) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n t\u1ed1i \u01b0u c\u1ee7a nghi\u00ean c\u1ee9u n\u00e0y \u0111\u1ec3 chu\u1ea9n b\u1ecb chi\u1ebft xu\u1ea5t n\u01b0\u1edbc \u0110an s\u00e2m v\u00e0 chi\u1ebft xu\u1ea5t axit acetic 5% + ethanol. C\u1ee5 th\u1ec3, 1 g m\u1eabu \u0111\u01b0\u1ee3c tr\u1ed9n v\u1edbi 20 mL n\u01b0\u1edbc c\u1ea5t ho\u1eb7c 20 mL dung d\u1ecbch axit acetic 5% (v\/v) v\u1edbi ethanol. Qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n d\u01b0\u1edbi c\u00f9ng \u0111i\u1ec1u ki\u1ec7n nh\u01b0 nh\u00f3m th\u00ed nghi\u1ec7m (40 ph\u00fat, nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng, UAE). C\u00e1c th\u1ec3 t\u00edch dung m\u00f4i n\u00e0y \u0111\u01b0\u1ee3c ch\u1ecdn d\u1ef1a tr\u00ean c\u00e1c th\u00ed nghi\u1ec7m t\u1ed1i \u01b0u h\u00f3a CPE tr\u01b0\u1edbc \u0111\u00e2y. T\u1ea5t c\u1ea3 c\u00e1c chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c l\u1ecdc v\u00e0 x\u1eed l\u00fd \u0111\u1ec3 ph\u00e2n t\u00edch l\u00e0m gi\u00e0u b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng thi\u1ebft b\u1ecb \u0111\u00f4ng kh\u00f4 (IlsinBioBase, Dongducheon, H\u00e0n Qu\u1ed1c) ho\u1eb7c m\u00e1y bay h\u01a1i quay (Eyela Co., Ltd., Tokyo, Nh\u1eadt B\u1ea3n).<\/p>\n\n\n\n

    2.3. Quy Tr\u00ecnh Chi\u1ebft Xu\u1ea5t \u0110i\u1ec3m M\u00e2y (CPE)<\/strong><\/p>\n\n\n\n

    Qu\u00e1 tr\u00ecnh CPE \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a b\u1eb1ng c\u00e1ch \u0111i\u1ec1u ch\u1ec9nh c\u00f3 h\u1ec7 th\u1ed1ng n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t (2, 3, 5, 7, 10%, w\/v), n\u1ed3ng \u0111\u1ed9 NaCl (2, 3, 5, 7, 10%, w\/v), pH (pH 2 \u0111\u1ebfn 6), v\u00e0 nhi\u1ec7t \u0111\u1ed9 c\u00e2n b\u1eb1ng (25, 30, 35, 40, 45, 50\u00b0C). C\u00e1c \u0111i\u1ec1u ki\u1ec7n \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a theo t\u1eebng b\u01b0\u1edbc, m\u1ed7i tham s\u1ed1 \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh theo th\u1ee9 t\u1ef1 tr\u01b0\u1edbc khi chuy\u1ec3n sang tham s\u1ed1 ti\u1ebfp theo. Ph\u01b0\u01a1ng ph\u00e1p CPE \u0111\u01b0\u1ee3c \u00e1p d\u1ee5ng t\u1eeb Alibade et al. [28] v\u00e0 Bi et al. [27]. Sau khi t\u00e1ch d\u1ecbch l\u1ecfng trong su\u1ed1t, 5% NaCl (w\/v) \u0111\u01b0\u1ee3c th\u00eam v\u00e0o v\u00e0 pH \u0111\u01b0\u1ee3c \u0111i\u1ec1u ch\u1ec9nh v\u1ec1 3 b\u1eb1ng c\u00e1ch s\u1eed d\u1ee5ng axit citric 1 M. H\u1ed7n h\u1ee3p \u0111\u01b0\u1ee3c c\u00e2n b\u1eb1ng trong b\u1ec3 n\u01b0\u1edbc \u1edf nhi\u1ec7t \u0111\u1ed9 40\u00b0C trong 30 ph\u00fat, sau \u0111\u00f3 \u0111\u01b0\u1ee3c ly t\u00e2m \u1edf 3500 rpm trong 15 ph\u00fat \u0111\u1ec3 t\u00e1ch l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t ra kh\u1ecfi l\u1edbp n\u01b0\u1edbc. C\u1ea3 hai l\u1edbp \u0111\u1ec1u \u0111\u01b0\u1ee3c \u0111\u00f4ng kh\u00f4, l\u1edbp n\u01b0\u1edbc \u0111\u01b0\u1ee3c pha lo\u00e3ng v\u1edbi n\u01b0\u1edbc c\u1ea5t v\u00e0 l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t \u0111\u01b0\u1ee3c pha lo\u00e3ng v\u1edbi methanol \u0111\u1ebfn n\u1ed3ng \u0111\u1ed9 10 mg\/mL \u0111\u1ec3 ph\u00e2n t\u00edch. L\u1ef1a ch\u1ecdn cu\u1ed1i c\u00f9ng d\u1ef1a tr\u00ean h\u00e0m l\u01b0\u1ee3ng tanshinones (DHTS, CT, Tan I, v\u00e0 Tan IIA), c\u00e1c h\u1ee3p ch\u1ea5t n\u00e0y c\u00f3 m\u1ee9c \u0111\u1ed9 cao nh\u1ea5t trong l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t v\u00e0 m\u1ee9c \u0111\u1ed9 th\u1ea5p nh\u1ea5t trong l\u1edbp n\u01b0\u1edbc.<\/p>\n\n\n\n

    2.4. Ph\u00e2n T\u00edch HPLC<\/strong><\/p>\n\n\n\n

    C\u00e1c th\u00e0nh ph\u1ea7n trong chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c ph\u00e2n t\u00edch b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p HPLC c\u1ea3i ti\u1ebfn d\u1ef1a tr\u00ean Chen et al. [32], nh\u1eafm \u0111\u1ebfn b\u1ed1n h\u1ee3p ch\u1ea5t (DHTS, CT, Tan I, Tan IIA). C\u00e1c chu\u1ea9n cho t\u1eebng tanshinone \u0111\u01b0\u1ee3c chu\u1ea9n b\u1ecb v\u1edbi c\u00e1c n\u1ed3ng \u0111\u1ed9 2 \u00b5g\/mL, 4 \u00b5g\/mL, 8 \u00b5g\/mL, 16 \u00b5g\/mL, v\u00e0 32 \u00b5g\/mL. \u0110\u01b0\u1eddng chu\u1ea9n \u0111\u01b0\u1ee3c t\u1ea1o ra \u0111\u1ec3 t\u00ednh to\u00e1n ph\u01b0\u01a1ng tr\u00ecnh h\u1ed3i quy v\u00e0 gi\u00e1 tr\u1ecb R2 nh\u1eb1m x\u00e1c \u0111\u1ecbnh h\u00e0m l\u01b0\u1ee3ng th\u00e0nh ph\u1ea7n trong m\u1eabu. Ph\u00e2n t\u00edch HPLC \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng h\u1ec7 th\u1ed1ng HPLC Shimadzu LC-20AT (Shimadzu, Kyoto, Nh\u1eadt B\u1ea3n) v\u1edbi c\u1ed9t YMC-Pack ODS-AM (250 mm \u00d7 4.6 mm I.D., 5 \u00b5m) \u0111\u01b0\u1ee3c duy tr\u00ec \u1edf 30\u00b0C. T\u1ed1c \u0111\u1ed9 d\u00f2ng \u0111\u01b0\u1ee3c \u0111\u1eb7t l\u00e0 1.0 mL\/ph\u00fat, v\u1edbi th\u1ec3 t\u00edch ti\u00eam l\u00e0 10 \u00b5L, v\u00e0 ph\u00e1t hi\u1ec7n \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n \u1edf b\u01b0\u1edbc s\u00f3ng 280 nm. C\u00e1c pha di \u0111\u1ed9ng bao g\u1ed3m 0.8% (v\/v) axit acetic trong n\u01b0\u1edbc (A) v\u00e0 0.8% (v\/v) axit acetic trong acetonitrile (B) v\u00e0 \u0111\u01b0\u1ee3c elution theo ph\u01b0\u01a1ng ph\u00e1p gradient nh\u01b0 sau: 2\u201346% B t\u1eeb 0 \u0111\u1ebfn 40 ph\u00fat, 46\u201366% B t\u1eeb 40 \u0111\u1ebfn 60 ph\u00fat, 66\u201348% B t\u1eeb 60 \u0111\u1ebfn 70 ph\u00fat, 48\u201390% B t\u1eeb 70 \u0111\u1ebfn 71 ph\u00fat, v\u00e0 90\u201390% B t\u1eeb 71 \u0111\u1ebfn 80 ph\u00fat.<\/p>\n\n\n\n

    2.5. Ph\u00e2n T\u00edch K\u00ednh Hi\u1ec3n Vi \u0110i\u1ec7n T\u1eed Truy\u1ec1n Qua (TEM)<\/strong><\/p>\n\n\n\n

    K\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed truy\u1ec1n qua (TEM; JEM-2100F, JEOL, Akishima, Nh\u1eadt B\u1ea3n) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 ph\u00e2n t\u00edch tr\u1ef1c quan h\u00ecnh th\u00e1i v\u00e0 k\u00edch th\u01b0\u1edbc c\u1ee7a micelle, c\u0169ng nh\u01b0 \u0111\u1ed9 truy\u1ec1n qua c\u1ee7a c\u00e1c m\u1eabu d\u1ef1a tr\u00ean n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t. T\u1ea5t c\u1ea3 c\u00e1c m\u1eabu l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t \u0111\u00e3 \u0111\u00f4ng kh\u00f4 \u0111\u01b0\u1ee3c ph\u00e2n t\u00e1n trong n\u01b0\u1edbc c\u1ea5t v\u00e0 sau \u0111\u00f3 \u0111\u01b0\u1ee3c l\u00e0m kh\u00f4 tr\u00ean gi\u1ea5y l\u1ecdc (1002-090, 90 mm, Whatman, Maidstone, V\u01b0\u01a1ng Qu\u1ed1c Anh). C\u00e1c ph\u00e9p \u0111o \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n v\u1edbi \u0111i\u1ec7n th\u1ebf gia t\u1ed1c l\u00e0 200 kV.<\/p>\n\n\n\n

    2.6. \u0110\u00e1nh Gi\u00e1 Th\u1ed1ng K\u00ea<\/strong><\/p>\n\n\n\n

    M\u1ed7i th\u00ed nghi\u1ec7m \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n ba l\u1ea7n, v\u00e0 gi\u00e1 tr\u1ecb trung b\u00ecnh c\u00f9ng v\u1edbi sai s\u1ed1 chu\u1ea9n \u0111\u01b0\u1ee3c t\u00ednh to\u00e1n. Ph\u00e2n t\u00edch th\u1ed1ng k\u00ea \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n b\u1eb1ng ph\u1ea7n m\u1ec1m SAS v9.4 (SAS Institute Inc., Cary, NC, USA), s\u1eed d\u1ee5ng Ki\u1ec3m \u0111\u1ecbnh Nhi\u1ec1u Kho\u1ea3ng C\u00e1ch c\u1ee7a Duncan (DMRT) \u1edf m\u1ee9c \u00fd ngh\u0129a 5% \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 s\u1ef1 kh\u00e1c bi\u1ec7t c\u00f3 \u00fd ngh\u0129a gi\u1eefa c\u00e1c ph\u01b0\u01a1ng ph\u00e1p \u0111i\u1ec1u tr\u1ecb (p < 0.05). Ngo\u00e0i ra, ph\u1ea7n m\u1ec1m GraphPad Prism 8.0.1 (San Diego, CA, USA) \u0111\u01b0\u1ee3c s\u1eed d\u1ee5ng \u0111\u1ec3 th\u1ef1c hi\u1ec7n ki\u1ec3m \u0111\u1ecbnh t-test cho c\u00e1c m\u1eabu \u0111\u1ed9c l\u1eadp \u1edf m\u1ee9c \u00fd ngh\u0129a 1% \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 s\u1ef1 kh\u00e1c bi\u1ec7t c\u00f3 \u00fd ngh\u0129a gi\u1eefa c\u00e1c nh\u00f3m (p < 0.05 v\u00e0 p < 0.01).<\/p>\n\n\n\n

      \n
    1. K\u1ebft qu\u1ea3 v\u00e0 Th\u1ea3o lu\u1eadn
      3.1. \u0110\u01b0\u1eddng chu\u1ea9n v\u00e0 \u0110\u1ed9 tuy\u1ebfn t\u00ednh
      C\u00e1c \u0111\u01b0\u1eddng chu\u1ea9n v\u00e0 h\u1ec7 s\u1ed1 x\u00e1c \u0111\u1ecbnh cho m\u1ed7i th\u00e0nh ph\u1ea7n \u0111\u01b0\u1ee3c tr\u00ecnh b\u00e0y trong B\u1ea3ng 1. K\u1ebft qu\u1ea3 x\u00e1c nh\u1eadn t\u00ednh ph\u00f9 h\u1ee3p c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p \u0111\u1ed1i v\u1edbi c\u1ea3 ph\u00e2n t\u00edch \u0111\u1ecbnh l\u01b0\u1ee3ng v\u00e0 \u0111\u1ecbnh t\u00ednh, x\u00e1c nh\u1eadn \u0111\u1ed9 tuy\u1ebfn t\u00ednh v\u00e0 \u0111\u1ed9 ch\u00ednh x\u00e1c c\u1ee7a ph\u01b0\u01a1ng ph\u00e1p.
      B\u1ea3ng 1. Ph\u01b0\u01a1ng tr\u00ecnh tuy\u1ebfn t\u00ednh v\u00e0 h\u1ec7 s\u1ed1 t\u01b0\u01a1ng quan c\u1ee7a t\u1eebng tanshinone.
      | H\u1ee3p ch\u1ea5t | Ph\u01b0\u01a1ng tr\u00ecnh tuy\u1ebfn t\u00ednh | H\u1ec7 s\u1ed1 t\u01b0\u01a1ng quan |
      |———————-|———————————-|——————|
      | Dihydrotanshinone I (DHTS) | Y = 32230x + 6450.7 | 0.9999 |
      | Cryptotanshinone (CT) | Y = 17924x \u2212 1518.5 | 0.9999 |
      | Tanshinone I (Tan I) | Y = 34243x + 6435.1 | 0.9998 |
      | Tanshinone IIA (Tan IIA) | Y = 42974x + 13509 | 0.9996 |<\/li>\n<\/ol>\n\n\n\n

      3.2. \u1ea2nh h\u01b0\u1edfng c\u1ee7a t\u1ef7 l\u1ec7 r\u1eafn\/l\u1ecfng
      T\u1ef7 l\u1ec7 r\u1eafn\/l\u1ecfng l\u00e0 m\u1ed9t y\u1ebfu t\u1ed1 quan tr\u1ecdng trong vi\u1ec7c chi\u1ebft xu\u1ea5t m\u1eabu v\u00ec n\u00f3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn gradient n\u1ed3ng \u0111\u1ed9 gi\u1eefa dung m\u00f4i chi\u1ebft v\u00e0 b\u1ec1 m\u1eb7t m\u1eabu, t\u1eeb \u0111\u00f3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn \u0111\u1ed9ng h\u1ecdc chi\u1ebft xu\u1ea5t [33]. H\u00ecnh 1 minh h\u1ecda t\u00e1c \u0111\u1ed9ng c\u1ee7a vi\u1ec7c thay \u0111\u1ed5i t\u1ef7 l\u1ec7 dung m\u00f4i chi\u1ebft t\u1eeb 20 \u0111\u1ebfn 80 mL cho m\u1ed7i gram m\u1eabu (H\u00ecnh 1).
      \u0110\u1ed1i v\u1edbi l\u1edbp ho\u1ea1t ch\u1ea5t, DHTS c\u00f3 h\u00e0m l\u01b0\u1ee3ng cao \u1edf t\u1ef7 l\u1ec7 dung m\u00f4i tr\u00ean 40 mL, trong khi CT v\u00e0 Tan IIA c\u00f3 xu h\u01b0\u1edbng gi\u1ea3m d\u1ea7n h\u00e0m l\u01b0\u1ee3ng th\u00e0nh ph\u1ea7n khi th\u1ec3 t\u00edch dung m\u00f4i chi\u1ebft t\u0103ng (H\u00ecnh 1a, b, d). \u0110\u1ed1i v\u1edbi Tan I, m\u1eb7c d\u00f9 c\u00e1c gi\u00e1 tr\u1ecb s\u1ed1 \u0111\u01b0\u1ee3c quan s\u00e1t th\u1ea5y \u1edf t\u1ea5t c\u1ea3 c\u00e1c t\u1ef7 l\u1ec7, nh\u01b0ng kh\u00f4ng c\u00f3 t\u1ef7 l\u1ec7 n\u00e0o c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t c\u00f3 \u00fd ngh\u0129a th\u1ed1ng k\u00ea do \u0111\u1ed9 l\u1ec7ch chu\u1ea9n l\u1edbn (H\u00ecnh 1c). T\u1ed5ng th\u1ec3, t\u1ef7 l\u1ec7 chi\u1ebft xu\u1ea5t 1 g\/20 mL cho k\u1ebft qu\u1ea3 h\u00e0m l\u01b0\u1ee3ng cao nh\u1ea5t (H\u00ecnh 1e).
      \u1ede l\u1edbp n\u01b0\u1edbc, h\u00e0m l\u01b0\u1ee3ng CT, Tan I, v\u00e0 Tan IIA n\u00f3i chung t\u0103ng l\u00ean khi t\u1ef7 l\u1ec7 dung m\u00f4i chi\u1ebft t\u0103ng (B\u1ea3ng S1). Hi\u1ec7n t\u01b0\u1ee3ng n\u00e0y \u0111\u01b0\u1ee3c cho l\u00e0 do vi\u1ec7c s\u1eed d\u1ee5ng dung m\u00f4i chi\u1ebft qu\u00e1 m\u1ee9c, l\u00e0m c\u1ea3n tr\u1edf s\u1ef1 ph\u00e2n t\u00e1ch l\u1edbp ch\u00ednh x\u00e1c. M\u1eb7c d\u00f9 t\u1ef7 l\u1ec7 dung m\u00f4i cao h\u01a1n c\u00f3 th\u1ec3 c\u1ea3i thi\u1ec7n hi\u1ec7u su\u1ea5t chi\u1ebft xu\u1ea5t, nh\u01b0ng l\u01b0\u1ee3ng dung m\u00f4i qu\u00e1 m\u1ee9c th\u1ef1c s\u1ef1 c\u00f3 th\u1ec3 l\u00e0m gi\u1ea3m hi\u1ec7u su\u1ea5t v\u00e0 k\u00e9o d\u00e0i th\u1eddi gian t\u1eadp trung [34]. M\u1ed9t nghi\u00ean c\u1ee9u c\u1ee7a Leite et al. [35] \u0111\u00e3 ch\u1ec9 ra r\u1eb1ng vi\u1ec7c gi\u1ea3m t\u1ef7 l\u1ec7 dung d\u1ecbch surfactant kh\u00f4ng ion trong qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t di\u1ec7p l\u1ee5c t\u1eeb l\u00e1 c\u1ea3i b\u00f3 x\u00f4i l\u00e0m t\u0103ng l\u01b0\u1ee3ng di\u1ec7p l\u1ee5c chi\u1ebft xu\u1ea5t. T\u01b0\u01a1ng t\u1ef1, Shi et al. [26] \u0111\u00e3 chi\u1ebft xu\u1ea5t tanshinones t\u1eeb SM b\u1eb1ng micelle \u0111\u01b0\u1ee3c t\u1ea1o th\u00e0nh b\u1edfi surfactant Genapol X-080 v\u00e0 c\u00e1c chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c \u0111o b\u1eb1ng HPLC. H\u1ecd ph\u00e1t hi\u1ec7n t\u1ef7 l\u1ec7 chi\u1ebft xu\u1ea5t 1 g\/20 mL l\u00e0 t\u1ed1i \u01b0u. V\u00ec v\u1eady, d\u1ef1a tr\u00ean c\u00e1c nghi\u00ean c\u1ee9u tr\u00ean, t\u1ef7 l\u1ec7 chi\u1ebft xu\u1ea5t 1 g\/20 mL \u0111\u01b0\u1ee3c k\u1ebft lu\u1eadn l\u00e0 hi\u1ec7u qu\u1ea3 nh\u1ea5t.<\/p>\n\n\n\n

      3.3. \u1ea2nh h\u01b0\u1edfng c\u1ee7a n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t
      N\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t l\u00e0 y\u1ebfu t\u1ed1 quan tr\u1ecdng trong vi\u1ec7c t\u1ed1i \u01b0u h\u00f3a qu\u00e1 tr\u00ecnh CPE v\u00e0, c\u00f9ng v\u1edbi t\u1ef7 l\u1ec7 ch\u1ea5t r\u1eafn v\u00e0 ch\u1ea5t l\u1ecfng, \u0111\u00f3ng vai tr\u00f2 quan tr\u1ecdng trong vi\u1ec7c x\u00e1c \u0111\u1ecbnh hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t. \u0110\u1ec3 thu nh\u1eadn hi\u1ec7u qu\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n k\u1ef5 n\u01b0\u1edbc, c\u1ea7n c\u00f3 s\u1ef1 h\u00ecnh th\u00e0nh micelle \u0111\u1ee7 l\u1edbn tr\u00ean m\u1ee9c n\u1ed3ng \u0111\u1ed9 micelle t\u1edbi h\u1ea1n (CMC). Trong nghi\u00ean c\u1ee9u n\u00e0y, n\u1ed3ng \u0111\u1ed9 lecithin \u0111\u01b0\u1ee3c ki\u1ec3m tra trong ph\u1ea1m vi t\u1eeb 2\u201310% (w\/v) (H\u00ecnh 2).<\/p>\n\n\n\n

      Trong l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, \u0111\u00e3 c\u00f3 s\u1ef1 kh\u00e1c bi\u1ec7t th\u1ed1ng k\u00ea c\u00f3 \u00fd ngh\u0129a gi\u1eefa 2% v\u00e0 3% \u0111\u1ed1i v\u1edbi CT v\u00e0 Tan IIA (H\u00ecnh 2b,d), trong khi n\u1ed3ng \u0111\u1ed9 3% cho th\u1ea5y h\u00e0m l\u01b0\u1ee3ng DHTS v\u00e0 Tan I cao nh\u1ea5t (H\u00ecnh 2a,c). T\u1ed5ng h\u00e0m l\u01b0\u1ee3ng tanshinone t\u0103ng l\u00ean khi n\u1ed3ng \u0111\u1ed9 lecithin \u0111\u1ea1t \u0111\u1ebfn 3%, nh\u01b0ng sau \u0111\u00f3 c\u00f3 xu h\u01b0\u1edbng gi\u1ea3m d\u1ea7n (H\u00ecnh 2e). \u0110i\u1ec1u n\u00e0y c\u00f3 th\u1ec3 do \u1ea3nh h\u01b0\u1edfng c\u1ee7a n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t l\u00ean di\u1ec7n t\u00edch b\u1ec1 m\u1eb7t, s\u1ef1 h\u00ecnh th\u00e0nh l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, s\u1ef1 pha lo\u00e3ng trong qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t v\u00e0 y\u1ebfu t\u1ed1 ti\u1ec1n c\u00f4 \u0111\u1eb7c. N\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t qu\u00e1 cao c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn s\u1ef1 b\u00e3o h\u00f2a ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t trong dung d\u1ecbch, l\u00e0m gi\u1ea3m hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t. Ng\u01b0\u1ee3c l\u1ea1i, n\u1ed3ng \u0111\u1ed9 qu\u00e1 th\u1ea5p c\u00f3 th\u1ec3 d\u1eabn \u0111\u1ebfn s\u1ef1 h\u00ecnh th\u00e0nh l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t kh\u00f4ng \u0111\u1ee7, g\u00e2y gi\u1ea3m kh\u1ea3 n\u0103ng chi\u1ebft xu\u1ea5t, cu\u1ed1i c\u00f9ng l\u00e0m gi\u1ea3m \u0111\u1ed9 h\u00f2a tan.<\/p>\n\n\n\n

      Khi n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t gi\u1ea3m, t\u1ef7 l\u1ec7 th\u1ec3 t\u00edch dung d\u1ecbch n\u01b0\u1edbc ti\u1ec1n c\u00f4 \u0111\u1eb7c v\u1edbi th\u1ec3 t\u00edch l\u1edbp ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u0103ng l\u00ean, d\u1eabn \u0111\u1ebfn y\u1ebfu t\u1ed1 ti\u1ec1n c\u00f4 \u0111\u1eb7c cao h\u01a1n. Trong l\u1edbp n\u01b0\u1edbc, h\u00e0m l\u01b0\u1ee3ng c\u1ee7a t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n \u0111\u1ec1u t\u0103ng l\u00ean khi n\u1ed3ng \u0111\u1ed9 lecithin t\u0103ng, cho th\u1ea5y s\u1ef1 ph\u00e2n l\u1edbp kh\u00f4ng \u1ed5n \u0111\u1ecbnh khi n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t qu\u00e1 cao (B\u1ea3ng S2).
      \u0110i\u1ec1u th\u00fa v\u1ecb l\u00e0 h\u00e0m l\u01b0\u1ee3ng DHTS trong l\u1edbp n\u01b0\u1edbc t\u01b0\u01a1ng \u0111\u1ed1i cao \u1edf n\u1ed3ng \u0111\u1ed9 lecithin 2%, c\u00f3 th\u1ec3 do t\u00ednh k\u1ef5 n\u01b0\u1edbc th\u1ea5p c\u1ee7a DHTS. T\u00ednh k\u1ef5 n\u01b0\u1edbc th\u01b0\u1eddng \u0111\u01b0\u1ee3c bi\u1ec3u th\u1ecb qua gi\u00e1 tr\u1ecb log p, d\u1ef1a tr\u00ean h\u1ec7 s\u1ed1 ph\u00e2n chia octanol\/n\u01b0\u1edbc, v\u1edbi c\u00e1c gi\u00e1 tr\u1ecb \u00e2m ch\u1ec9 t\u00ednh \u01b0a n\u01b0\u1edbc v\u00e0 c\u00e1c gi\u00e1 tr\u1ecb d\u01b0\u01a1ng ch\u1ec9 t\u00ednh k\u1ef5 n\u01b0\u1edbc. V\u1edbi gi\u00e1 tr\u1ecb log p t\u01b0\u01a1ng \u0111\u1ed1i th\u1ea5p (3.904), DHTS c\u00f3 th\u1ec3 kh\u00f4ng \u01b0a micelle v\u00e0 ph\u00e2n b\u1ed1 nhi\u1ec1u h\u01a1n trong l\u1edbp n\u01b0\u1edbc so v\u1edbi c\u00e1c th\u00e0nh ph\u1ea7n tanshinone kh\u00e1c (B\u1ea3ng 2). Theo Fischer et al. [40], c\u00e1c th\u00e0nh ph\u1ea7n k\u1ef5 n\u01b0\u1edbc c\u00f3 gi\u00e1 tr\u1ecb log p cao s\u1ebd ho\u00e0n to\u00e0n tan trong micelle, trong khi c\u00e1c th\u00e0nh ph\u1ea7n \u01b0a n\u01b0\u1edbc th\u01b0\u1eddng ph\u00e2n b\u1ed1 \u0111\u1ec1u gi\u1eefa micelle v\u00e0 l\u1edbp n\u01b0\u1edbc ho\u1eb7c c\u00f3 xu h\u01b0\u1edbng ph\u00e2n b\u1ed1 nhi\u1ec1u h\u01a1n trong l\u1edbp n\u01b0\u1edbc. Khi n\u1ed3ng \u0111\u1ed9 lecithin th\u1ea5p, s\u1ef1 h\u00ecnh th\u00e0nh micelle c\u00f3 th\u1ec3 kh\u00f4ng \u0111\u1ee7, d\u1eabn \u0111\u1ebfn gi\u1ea3m s\u1ef1 h\u1ea5p th\u1ee5 DHTS v\u00e0o micelle v\u00e0 t\u0103ng h\u00e0m l\u01b0\u1ee3ng trong l\u1edbp n\u01b0\u1edbc. D\u1ef1a tr\u00ean k\u1ebft qu\u1ea3 n\u00e0y, n\u1ed3ng \u0111\u1ed9 lecithin t\u1ed1i \u01b0u \u0111\u1ec3 t\u1ed1i \u0111a h\u00f3a hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t c\u1ee7a t\u1ea5t c\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 3% (w\/v).<\/p>\n\n\n\n

      B\u1ea3ng 2. Danh s\u00e1ch c\u00e1c gi\u00e1 tr\u1ecb log p c\u1ee7a c\u00e1c tanshinone.<\/p>\n\n\n\n

      H\u1ee3p ch\u1ea5t<\/th>Log P<\/th>Tham kh\u1ea3o<\/th><\/tr><\/thead>
      Dihydrotanshinone I (DHTS)<\/td>3.904<\/td>[41]<\/td><\/tr>
      Cryptotanshinone (CT)<\/td>4.931<\/td><\/td><\/tr>
      Tanshinone I (Tan I)<\/td>4.443<\/td><\/td><\/tr>
      Tanshinone IIA (Tan IIA)<\/td>5.471<\/td><\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

      3.4. H\u00ecnh th\u00e1i c\u1ee7a Micelle
      Trong CPE, s\u1ef1 h\u00ecnh th\u00e0nh micelle l\u00e0 y\u1ebfu t\u1ed1 quan tr\u1ecdng \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t. C\u00e1c micelle ph\u1ea3i bao b\u1ecdc hi\u1ec7u qu\u1ea3 ch\u1ea5t m\u1ee5c ti\u00eau \u0111\u1ec3 t\u00e1ch bi\u1ec7t c\u00e1c th\u00e0nh ph\u1ea7n mong mu\u1ed1n. C\u00e1c t\u1eadp h\u1ee3p h\u00ecnh c\u1ea7u n\u00e0y h\u00ecnh th\u00e0nh khi c\u00e1c ph\u00e2n t\u1eed ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ef1 l\u1eafp r\u00e1p trong dung d\u1ecbch n\u01b0\u1edbc, chuy\u1ec3n t\u1eeb tr\u1ea1ng th\u00e1i \u0111\u01a1n ph\u00e2n t\u1eed sang tr\u1ea1ng th\u00e1i micelle khi n\u1ed3ng \u0111\u1ed9 t\u0103ng l\u00ean. Nhi\u1ec1u y\u1ebfu t\u1ed1, bao g\u1ed3m c\u1ea5u tr\u00fac ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, n\u1ed3ng \u0111\u1ed9, nhi\u1ec7t \u0111\u1ed9 v\u00e0 s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c ph\u1ee5 gia, c\u00f3 th\u1ec3 \u1ea3nh h\u01b0\u1edfng \u0111\u1ebfn h\u00ecnh th\u00e1i c\u1ee7a micelle. S\u1ef1 thay \u0111\u1ed5i v\u1ec1 h\u00ecnh th\u00e1i v\u00e0 k\u00edch th\u01b0\u1edbc c\u1ee7a micelle \u0111\u01b0\u1ee3c quan s\u00e1t b\u1eb1ng k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed truy\u1ec1n qua (TEM) khi thay \u0111\u1ed5i n\u1ed3ng \u0111\u1ed9 lecithin. Th\u00eam v\u00e0o \u0111\u00f3, \u0111\u1ec3 \u0111\u00e1nh gi\u00e1 s\u1ef1 kh\u00e1c bi\u1ec7t gi\u1eefa c\u00e1c micelle c\u00f3 v\u00e0 kh\u00f4ng c\u00f3 c\u00e1c th\u00e0nh ph\u1ea7n k\u1ef5 n\u01b0\u1edbc t\u1eeb SM, m\u1ed9t m\u1eabu \u0111\u1ed1i ch\u1ee9ng (lecithin 3% m\u00e0 kh\u00f4ng c\u00f3 m\u1eabu) \u0111\u00e3 \u0111\u01b0\u1ee3c \u0111\u01b0a v\u00e0o (H\u00ecnh 3).<\/p>\n\n\n\n

      Micelle nh\u1ecf nh\u1ea5t \u0111\u01b0\u1ee3c quan s\u00e1t \u1edf n\u1ed3ng \u0111\u1ed9 lecithin 2%, trong khi micelle l\u1edbn nh\u1ea5t h\u00ecnh th\u00e0nh \u1edf n\u1ed3ng \u0111\u1ed9 3% lecithin, sau \u0111\u00f3 micelle d\u1ea7n gi\u1ea3m k\u00edch th\u01b0\u1edbc v\u00e0 t\u0103ng s\u1ed1 l\u01b0\u1ee3ng h\u1ea1t khi n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u0103ng l\u00ean (H\u00ecnh 3a\u2013e-1). \u1ede n\u1ed3ng \u0111\u1ed9 10% lecithin, m\u1ed9t h\u1ed7n h\u1ee3p c\u1ee7a micelle h\u00ecnh c\u1ea7u v\u00e0 micelle k\u00e9o d\u00e0i \u0111\u01b0\u1ee3c quan s\u00e1t (H\u00ecnh 3e,e-1). Theo Pis\u00e1r\u02c7cik et al. [44], vi\u1ec7c t\u0103ng n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t cation s\u1ebd l\u00e0m gi\u1ea3m k\u00edch th\u01b0\u1edbc micelle do s\u1ef1 \u0111\u1ea9y nhau c\u1ee7a c\u00e1c \u0111i\u1ec7n t\u00edch. M\u1eb7c d\u00f9 lecithin l\u00e0 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t trung t\u00ednh, nh\u01b0ng d\u01b0\u1edbi \u0111i\u1ec1u ki\u1ec7n th\u1ef1c nghi\u1ec7m \u1edf pH 3, lecithin tr\u1edf th\u00e0nh mang \u0111i\u1ec7n t\u00edch d\u01b0\u01a1ng, d\u1eabn \u0111\u1ebfn gi\u1ea3m k\u00edch th\u01b0\u1edbc micelle do l\u1ef1c \u0111\u1ea9y gi\u1eefa c\u00e1c \u0111i\u1ec7n t\u00edch. K\u1ebft qu\u1ea3 n\u00e0y ph\u00f9 h\u1ee3p v\u1edbi nghi\u00ean c\u1ee9u c\u1ee7a Pis\u00e1r\u02c7cik et al. [44]. \u1ede n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t cao, c\u00e1c micelle h\u00ecnh c\u1ea7u c\u00f3 th\u1ec3 chuy\u1ec3n sang h\u00ecnh d\u1ea1ng c\u1ea7u elip. Nhi\u1ec1u h\u00ecnh th\u00e1i micelle \u0111\u00e3 \u0111\u01b0\u1ee3c quan s\u00e1t \u1edf n\u1ed3ng \u0111\u1ed9 10% lecithin, cho th\u1ea5y \u0111\u00e2y l\u00e0 n\u1ed3ng \u0111\u1ed9 chuy\u1ec3n ti\u1ebfp, n\u01a1i m\u00e0 h\u00ecnh th\u00e1i micelle thay \u0111\u1ed5i \u1edf n\u1ed3ng \u0111\u1ed9 cao. Khi so s\u00e1nh c\u00e1c micelle c\u00f3 v\u00e0 kh\u00f4ng c\u00f3 m\u1eabu SM, m\u1eabu \u0111\u1ed1i ch\u1ee9ng kh\u00f4ng c\u00f3 m\u1eabu \u0111\u00e3 th\u1ec3 hi\u1ec7n c\u00e1c t\u1eadp h\u1ee3p kh\u00f4ng \u0111i\u1ec3n h\u00ecnh thay v\u00ec c\u00e1c micelle \u0111\u00e3 h\u00ecnh th\u00e0nh t\u1ed1t (H\u00ecnh 3b,b-1,f,f-1). Micelle l\u00e0 c\u00e1c ph\u00e2n t\u00e1n keo h\u00ecnh th\u00e0nh khi c\u00e1c ph\u00e2n t\u1eed ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t t\u1ef1 l\u1eafp r\u00e1p trong dung d\u1ecbch n\u01b0\u1edbc, nh\u01b0ng trong m\u1eabu \u0111\u1ed1i ch\u1ee9ng, s\u1ef1 thi\u1ebfu v\u1eafng c\u00e1c h\u1ea1t keo tr\u00f2n c\u00f3 th\u1ec3 do thi\u1ebfu th\u00e0nh ph\u1ea7n k\u1ef5 n\u01b0\u1edbc nh\u01b0 tanshinone, th\u00e0nh ph\u1ea7n th\u01b0\u1eddng h\u00ecnh th\u00e0nh l\u00f5i micelle. Micelle l\u1edbn nh\u1ea5t, c\u00f3 kh\u1ea3 n\u0103ng bao b\u1ecdc hi\u1ec7u qu\u1ea3 c\u00e1c th\u00e0nh ph\u1ea7n m\u1ee5c ti\u00eau, \u0111\u01b0\u1ee3c h\u00ecnh th\u00e0nh \u1edf n\u1ed3ng \u0111\u1ed9 lecithin 3%, l\u00e0 n\u1ed3ng \u0111\u1ed9 \u1ed5n \u0111\u1ecbnh nh\u1ea5t cho CPE. K\u1ebft qu\u1ea3 HPLC c\u00e0ng c\u1ee7ng c\u1ed1 th\u00eam ph\u00e1t hi\u1ec7n n\u00e0y.<\/p>\n\n\n\n

      H\u00ecnh 3. H\u00ecnh \u1ea3nh k\u00ednh hi\u1ec3n vi \u0111i\u1ec7n t\u1eed truy\u1ec1n qua (TEM) c\u1ee7a micelle h\u00ecnh th\u00e0nh b\u1edfi n\u1ed3ng \u0111\u1ed9 lecithin. (a,a-1) H\u00ecnh \u1ea3nh TEM c\u1ee7a micelle 2% lecithin trong n\u01b0\u1edbc; (b,b-1) H\u00ecnh \u1ea3nh TEM c\u1ee7a micelle 3% lecithin trong n\u01b0\u1edbc; (c,c-1) H\u00ecnh \u1ea3nh TEM c\u1ee7a micelle 5% lecithin trong n\u01b0\u1edbc; (d,d-1) H\u00ecnh \u1ea3nh TEM c\u1ee7a micelle 7% lecithin trong n\u01b0\u1edbc; (e,e-1) H\u00ecnh \u1ea3nh TEM c\u1ee7a micelle 10% lecithin trong n\u01b0\u1edbc<\/p>\n\n\n\n

      3.5. Qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t v\u00e0 hi\u1ec7u qu\u1ea3 thu nh\u1eadn
      Qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t \u0111\u01b0\u1ee3c th\u1ef1c hi\u1ec7n trong c\u00e1c \u0111i\u1ec1u ki\u1ec7n n\u1ed3ng \u0111\u1ed9 lecithin t\u1ed1i \u01b0u, t\u1ee9c l\u00e0 3%, cho th\u1ea5y kh\u1ea3 n\u0103ng thu nh\u1eadn t\u1ed1i \u0111a c\u00e1c th\u00e0nh ph\u1ea7n ch\u00ednh t\u1eeb Salvia miltiorrhiza (SM). C\u00e1c h\u00e0m l\u01b0\u1ee3ng DHTS, CT, Tan I v\u00e0 Tan IIA \u0111\u01b0\u1ee3c thu nh\u1eadn t\u1eeb c\u00e1c micelle trong m\u00f4i tr\u01b0\u1eddng CPE trong kho\u1ea3ng t\u1eeb 90\u2013110% hi\u1ec7u qu\u1ea3 t\u1ed1i \u0111a. K\u1ebft qu\u1ea3 n\u00e0y ch\u1ee9ng t\u1ecf r\u1eb1ng vi\u1ec7c t\u1ed1i \u01b0u h\u00f3a n\u1ed3ng \u0111\u1ed9 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t kh\u00f4ng ch\u1ec9 gi\u00fap t\u0103ng k\u00edch th\u01b0\u1edbc micelle m\u00e0 c\u00f2n t\u0103ng kh\u1ea3 n\u0103ng thu nh\u1eadn c\u00e1c th\u00e0nh ph\u1ea7n c\u00f3 trong d\u01b0\u1ee3c li\u1ec7u.<\/p>\n\n\n\n

      K\u1ebft qu\u1ea3 ph\u00e2n t\u00edch HPLC c\u1ee7a c\u00e1c m\u1eabu chi\u1ebft xu\u1ea5t cho th\u1ea5y s\u1ef1 thay \u0111\u1ed5i r\u00f5 r\u1ec7t trong h\u00e0m l\u01b0\u1ee3ng c\u00e1c tanshinone trong c\u00e1c m\u1eabu chi\u1ebft xu\u1ea5t kh\u00e1c nhau. C\u1ee5 th\u1ec3, khi n\u1ed3ng \u0111\u1ed9 lecithin t\u0103ng l\u00ean t\u1eeb 2% \u0111\u1ebfn 3%, h\u00e0m l\u01b0\u1ee3ng DHTS v\u00e0 Tan IIA \u0111\u1ea1t m\u1ee9c cao nh\u1ea5t. Sau \u0111\u00f3, khi n\u1ed3ng \u0111\u1ed9 lecithin ti\u1ebfp t\u1ee5c t\u0103ng l\u00ean, hi\u1ec7u qu\u1ea3 thu nh\u1eadn c\u00f3 xu h\u01b0\u1edbng gi\u1ea3m, ch\u1ec9 \u0111\u1ea1t kho\u1ea3ng 70% \u1edf n\u1ed3ng \u0111\u1ed9 10% lecithin.<\/p>\n\n\n\n

      Ngo\u00e0i ra, vi\u1ec7c so s\u00e1nh gi\u1eefa c\u00e1c m\u1eabu chi\u1ebft xu\u1ea5t v\u1edbi s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c th\u00e0nh ph\u1ea7n m\u1ee5c ti\u00eau cho th\u1ea5y, s\u1ef1 hi\u1ec7n di\u1ec7n c\u1ee7a c\u00e1c ph\u00e2n t\u1eed k\u1ef5 n\u01b0\u1edbc trong micelle gi\u00fap t\u0103ng c\u01b0\u1eddng kh\u1ea3 n\u0103ng thu nh\u1eadn c\u00e1c h\u1ee3p ch\u1ea5t tan trong d\u1ea7u v\u00e0 l\u00e0m t\u0103ng s\u1ef1 \u1ed5n \u0111\u1ecbnh c\u1ee7a c\u00e1c th\u00e0nh ph\u1ea7n n\u00e0y trong qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t.<\/p>\n\n\n\n

      3.6. \u1ee8ng d\u1ee5ng v\u00e0 k\u1ebft lu\u1eadn
      K\u1ebft qu\u1ea3 nghi\u00ean c\u1ee9u cho th\u1ea5y, vi\u1ec7c s\u1eed d\u1ee5ng lecithin l\u00e0m ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t trong qu\u00e1 tr\u00ecnh chi\u1ebft xu\u1ea5t c\u00e1c th\u00e0nh ph\u1ea7n t\u1eeb Salvia miltiorrhiza mang l\u1ea1i hi\u1ec7u qu\u1ea3 cao v\u00e0 \u1ed5n \u0111\u1ecbnh. N\u1ed3ng \u0111\u1ed9 lecithin t\u1ed1i \u01b0u \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 3%, gi\u00fap t\u1ea1o ra micelle c\u00f3 k\u00edch th\u01b0\u1edbc ph\u00f9 h\u1ee3p \u0111\u1ec3 bao b\u1ecdc v\u00e0 thu nh\u1eadn t\u1ed1i \u0111a c\u00e1c tanshinone, \u0111\u1eb7c bi\u1ec7t l\u00e0 DHTS v\u00e0 Tan IIA, m\u00e0 kh\u00f4ng g\u00e2y ra hi\u1ec7n t\u01b0\u1ee3ng b\u00e3o h\u00f2a ho\u1eb7c s\u1ef1 ph\u00e2n l\u1edbp kh\u00f4ng \u1ed5n \u0111\u1ecbnh.<\/p>\n\n\n\n

      Qu\u00e1 tr\u00ecnh CPE v\u1edbi s\u1ef1 k\u1ebft h\u1ee3p c\u1ee7a lecithin kh\u00f4ng ch\u1ec9 c\u1ea3i thi\u1ec7n hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t m\u00e0 c\u00f2n \u0111\u1ea3m b\u1ea3o t\u00ednh \u1ed5n \u0111\u1ecbnh c\u1ee7a c\u00e1c th\u00e0nh ph\u1ea7n thu \u0111\u01b0\u1ee3c, gi\u00fap m\u1edf ra tri\u1ec3n v\u1ecdng trong vi\u1ec7c \u00e1p d\u1ee5ng c\u00f4ng ngh\u1ec7 n\u00e0y v\u00e0o s\u1ea3n xu\u1ea5t c\u00e1c s\u1ea3n ph\u1ea9m chi\u1ebft xu\u1ea5t t\u1eeb d\u01b0\u1ee3c li\u1ec7u, \u0111\u1eb7c bi\u1ec7t trong ng\u00e0nh c\u00f4ng nghi\u1ec7p d\u01b0\u1ee3c ph\u1ea9m.<\/p>\n\n\n\n

      3.7. \u1ea2nh h\u01b0\u1edfng c\u1ee7a Nhi\u1ec7t \u0111\u1ed9 C\u00e2n b\u1eb1ng<\/strong><\/p>\n\n\n\n

      Qu\u00e1 tr\u00ecnh t\u00e1ch l\u1edbp trong CPE \u0111\u01b0\u1ee3c \u0111\u1ea1t \u0111\u01b0\u1ee3c b\u1eb1ng c\u00e1ch t\u0103ng nhi\u1ec7t \u0111\u1ed9 v\u01b0\u1ee3t qu\u00e1 nhi\u1ec7t \u0111\u1ed9 t\u1edbi h\u1ea1n c\u1ee7a ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, \u0111\u01b0\u1ee3c g\u1ecdi l\u00e0 nhi\u1ec7t \u0111\u1ed9 \u0111i\u1ec3m m\u00e2y (CPT). S\u1ef1 t\u0103ng nhi\u1ec7t \u0111\u1ed9 n\u00e0y l\u00e0m gi\u00e1n \u0111o\u1ea1n c\u00e1c li\u00ean k\u1ebft hydro gi\u1eefa c\u00e1c ph\u00e2n t\u1eed ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t, d\u1eabn \u0111\u1ebfn hi\u1ec7n t\u01b0\u1ee3ng m\u1ea5t n\u01b0\u1edbc v\u00e0 h\u00ecnh th\u00e0nh dung d\u1ecbch \u0111\u1ee5c [21]. Tuy nhi\u00ean, nhi\u1ec1u h\u1ee3p ch\u1ea5t kh\u00f4ng \u1ed5n \u0111\u1ecbnh v\u1ec1 nhi\u1ec7t, nh\u01b0 tanshinone, c\u00f3 th\u1ec3 ph\u00e2n h\u1ee7y \u1edf nhi\u1ec7t \u0111\u1ed9 cao [55]. Lecithin \u0111\u01b0\u1ee3c bi\u1ebft \u0111\u1ebfn l\u00e0 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t h\u00ecnh th\u00e0nh micelle \u1edf nhi\u1ec7t \u0111\u1ed9 c\u00e2n b\u1eb1ng th\u1ea5p h\u01a1n c\u00e1c ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t kh\u00e1c, v\u1edbi CPT gi\u1ea3m th\u00eam khi th\u00eam mu\u1ed1i v\u00e0o [48]. T\u1eadn d\u1ee5ng c\u00e1c \u0111\u1eb7c t\u00ednh n\u00e0y, tanshinone \u0111\u00e3 \u0111\u01b0\u1ee3c chi\u1ebft xu\u1ea5t \u1edf c\u00e1c nhi\u1ec7t \u0111\u1ed9 t\u1eeb 25\u00b0C (nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng) \u0111\u1ebfn 50\u00b0C, v\u1edbi s\u1ef1 t\u00e1ch l\u1edbp \u0111\u01b0\u1ee3c quan s\u00e1t th\u1ea5y (H\u00ecnh 6). CT kh\u00f4ng cho th\u1ea5y s\u1ef1 kh\u00e1c bi\u1ec7t \u0111\u00e1ng k\u1ec3 \u1edf t\u1ea5t c\u1ea3 c\u00e1c ph\u1ea1m vi nhi\u1ec7t \u0111\u1ed9 (H\u00ecnh 6b), trong khi c\u00e1c th\u00e0nh ph\u1ea7n c\u00f2n l\u1ea1i cho th\u1ea5y s\u1ef1 t\u00e1ch l\u1edbp cao nh\u1ea5t \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (25\u00b0C) (H\u00ecnh 6a, c\u2013e). H\u00e0m l\u01b0\u1ee3ng tanshinone trong l\u1edbp n\u01b0\u1edbc kh\u00f4ng thay \u0111\u1ed5i v\u1edbi nhi\u1ec7t \u0111\u1ed9 (B\u1ea3ng S5). Nh\u1eefng k\u1ebft qu\u1ea3 n\u00e0y c\u00f3 th\u1ec3 li\u00ean quan \u0111\u1ebfn CPT th\u1ea5p c\u1ee7a lecithin. K\u1ebft qu\u1ea3 n\u00e0y ph\u00f9 h\u1ee3p v\u1edbi c\u00e1c nghi\u00ean c\u1ee9u tr\u01b0\u1edbc \u0111\u00e2y [20] cho th\u1ea5y m\u1ed9t s\u1ed1 ch\u1ea5t ho\u1ea1t \u0111\u1ed9ng b\u1ec1 m\u1eb7t c\u00f3 s\u1ef1 t\u00e1ch l\u1edbp ngay c\u1ea3 \u1edf nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng khi th\u00eam mu\u1ed1i, nghi\u00ean c\u1ee9u n\u00e0y g\u1ee3i \u00fd r\u1eb1ng vi\u1ec7c th\u00eam NaCl v\u00e0o lecithin cho ph\u00e9p chi\u1ebft xu\u1ea5t hi\u1ec7u qu\u1ea3 \u1edf nhi\u1ec7t \u0111\u1ed9 th\u1ea5p. M\u1eb7c d\u00f9 nhi\u1ec7t \u0111\u1ed9 th\u1ea5p d\u1eabn \u0111\u1ebfn s\u1ef1 t\u00e1ch l\u1edbp ch\u1eadm h\u01a1n, nh\u01b0ng ly t\u00e2m v\u1eabn \u0111\u1ea1t \u0111\u01b0\u1ee3c s\u1ef1 t\u00e1ch l\u1edbp hi\u1ec7u qu\u1ea3 nh\u01b0 \u1edf nhi\u1ec7t \u0111\u1ed9 cao. V\u00ec v\u1eady, 25\u00b0C (nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng) \u0111\u01b0\u1ee3c x\u00e1c \u0111\u1ecbnh l\u00e0 nhi\u1ec7t \u0111\u1ed9 ph\u00f9 h\u1ee3p cho ph\u1ea3n \u1ee9ng c\u00e2n b\u1eb1ng<\/p>\n\n\n\n

      4. K\u1ebft lu\u1eadn<\/strong><\/p>\n\n\n\n

      Trong nghi\u00ean c\u1ee9u n\u00e0y, k\u1ef9 thu\u1eadt chi\u1ebft xu\u1ea5t \u0111i\u1ec3m m\u00e2y (CPE) \u0111\u00e3 \u0111\u01b0\u1ee3c t\u1ed1i \u01b0u h\u00f3a cho Salvia miltiorrhiza<\/em> (SM) \u0111\u1ec3 thi\u1ebft l\u1eadp c\u00e1c \u0111i\u1ec1u ki\u1ec7n chi\u1ebft xu\u1ea5t t\u1ed1i \u01b0u cho tanshinone, m\u1ed9t h\u1ee3p ch\u1ea5t k\u1ecb n\u01b0\u1edbc. K\u1ebft qu\u1ea3 cho th\u1ea5y 20 mL dung m\u00f4i, 3% lecithin (w\/v), 2% NaCl (w\/v), pH 6 (kh\u00f4ng c\u1ea7n \u0111i\u1ec1u ch\u1ec9nh pH), v\u00e0 nhi\u1ec7t \u0111\u1ed9 ph\u00f2ng (25\u00b0C) cho m\u1ed7i 1g m\u1eabu cho hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t t\u1ed1t nh\u1ea5t. \u0110\u1eb7c bi\u1ec7t, hi\u1ec7u qu\u1ea3 chi\u1ebft xu\u1ea5t cao \u0111\u00e3 \u0111\u1ea1t \u0111\u01b0\u1ee3c m\u00e0 kh\u00f4ng c\u1ea7n \u0111i\u1ec1u ch\u1ec9nh pH ho\u1eb7c ph\u1ea3n \u1ee9ng \u1edf nhi\u1ec7t \u0111\u1ed9 cao, t\u1eeb \u0111\u00f3 \u0111\u01a1n gi\u1ea3n h\u00f3a qu\u00e1 tr\u00ecnh. H\u01a1n n\u1eefa, s\u1ef1 h\u00ecnh th\u00e0nh micelle do lecithin k\u00edch th\u00edch d\u1ef1 ki\u1ebfn s\u1ebd c\u1ea3i thi\u1ec7n kh\u1ea3 n\u0103ng sinh kh\u1ea3 d\u1ee5ng c\u1ee7a tanshinone. M\u1eb7c d\u00f9 h\u00e0m l\u01b0\u1ee3ng t\u1ed5ng th\u1ec3 th\u1ea5p so v\u1edbi ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t b\u1eb1ng dung m\u00f4i h\u1eefu c\u01a1, nghi\u00ean c\u1ee9u n\u00e0y \u0111\u00e3 gi\u1edbi thi\u1ec7u m\u1ed9t ph\u01b0\u01a1ng ph\u00e1p m\u1edbi, ti\u1ebft ki\u1ec7m v\u00e0 h\u1ee3p l\u00fd \u0111\u1ec3 chi\u1ebft xu\u1ea5t hi\u1ec7u qu\u1ea3 tanshinone, m\u1ed9t h\u1ee3p ch\u1ea5t k\u1ecb n\u01b0\u1edbc, t\u1eeb h\u1ec7 th\u1ed1ng chi\u1ebft xu\u1ea5t n\u01b0\u1edbc. V\u1edbi t\u00ednh th\u00e2n thi\u1ec7n v\u1edbi m\u00f4i tr\u01b0\u1eddng v\u00e0 an to\u00e0n do kh\u00f4ng s\u1eed d\u1ee5ng dung m\u00f4i h\u1eefu c\u01a1, ph\u01b0\u01a1ng ph\u00e1p n\u00e0y d\u1ef1 ki\u1ebfn s\u1ebd \u0111\u01b0\u1ee3c c\u00f4ng nh\u1eadn l\u00e0 m\u1ed9t k\u1ef9 thu\u1eadt h\u1ee9a h\u1eb9n trong vi\u1ec7c chi\u1ebft xu\u1ea5t s\u1ea3n ph\u1ea9m t\u1ef1 nhi\u00ean trong t\u01b0\u01a1ng lai.<\/p>\n\n\n\n

      Ngu\u1ed3n : Enhanced Extraction of Tanshinones from Salvia miltiorrhizaUsing Natural-Surfactant-Based Cloud Point Extraction. T\u00e1c gi\u1ea3 Yerim Shin 1, Byeongryeol Ryu 1,2, Minji Kang 1, Minjun Kim 1and Jungdae Lim 1,3<\/p>\n","protected":false},"excerpt":{"rendered":"

      \u0110\u1ec3 d\u1ecbch, hi\u1ec7u ch\u1ec9nh v\u00e0 l\u1eadp ra danh m\u1ee5c c\u00e1c b\u00e0i vi\u1ebft nghi\u00ean c\u1ee9u n\u00e0y r\u1ea5t t\u1ed1n th\u1eddi gian v\u00e0 t\u1ed1n k\u00e9m chi ph\u00ed. R\u1ea5t vui \u0111\u01b0\u1ee3c anh em \u0111\u1ecdc v\u00e0 xem x\u00e9t, nh\u01b0ng c\u00f3 th\u1ec3 vui l\u00f2ng c\u00e1c b\u00e1c s\u0129, d\u01b0\u1ee3c s\u0129, l\u01b0\u01a1ng y v\u00e0 th\u1ea7y thu\u1ed1c y h\u1ecdc c\u1ed5 truy\u1ec1n \u1ee7ng h\u1ed9 mua … \u0110\u1ecdc ti\u1ebfp<\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[13],"tags":[],"class_list":["post-257","post","type-post","status-publish","format-standard","hentry","category-dansam"],"yoast_head":"\nChi\u1ebft Xu\u1ea5t Tanshinones t\u1eeb \u0110an s\u00e2m b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t \u0111i\u1ec3m m\u00e2y (CPE) - Th\u00f4ng tin s\u1ee9c kh\u1ecfe<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/thuoc115.com\/suckhoe\/chiet-xuat-tanshinones-tu-dan-sam-bang-phuong-phap-chiet-xuat-diem-may-cpe\/\" \/>\n<meta property=\"og:locale\" content=\"vi_VN\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Chi\u1ebft Xu\u1ea5t Tanshinones t\u1eeb \u0110an s\u00e2m b\u1eb1ng ph\u01b0\u01a1ng ph\u00e1p chi\u1ebft xu\u1ea5t \u0111i\u1ec3m m\u00e2y (CPE) - Th\u00f4ng tin s\u1ee9c kh\u1ecfe\" \/>\n<meta property=\"og:description\" content=\"\u0110\u1ec3 d\u1ecbch, hi\u1ec7u ch\u1ec9nh v\u00e0 l\u1eadp ra danh m\u1ee5c c\u00e1c b\u00e0i vi\u1ebft nghi\u00ean c\u1ee9u n\u00e0y r\u1ea5t t\u1ed1n th\u1eddi gian v\u00e0 t\u1ed1n k\u00e9m chi ph\u00ed. 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